12-301 | Non-Stimulated A431 Cell Lysate

Details:

Modified ripa buffer (50mm tris-hcl, ph 7.4, 1% Np40, 0.25% Sodium deoxycholate, 150mm nacl, 1mm edta, 1mm pmsf, 1μg/ml each of aprotinin, leupeptin, pepstatin, 1mm na3vo4, 1mm naf) and diluted with non-reducing sample buffer (31mm tris-hcl, ph 6.8, 5% Glycerol, 1% sds, 0.002% Bromphenol blue).