Acetylcholinesterase Type V-S From Eel, 5 Ku, Each

Details:

Specifications synonyms: ache, acetylcholine acetylhydrolase, cholinesterase, acetyl cas no.: 9000-81-1 Analysis note: the activity obtained using acetylcholine as substrate is 30-100 times that obtained with butyrylcholine, using acetylcholinesterase from electric eel. Biochem/physiol actions: major degradative enzyme for acetylcholine in vivo. Converts acetylcholine h2o to choline acetic acid. Caution: for dilute solutions (<1 mg l), add 1 mg l bsa to stabilize. Solutions containing at least 1 mg protein l in dilute neutral phosphate buffer are stable at least 6 months refrigerated. Unit definition: one unit will hydrolyze 1.0 µMole of acetylcholine to choline and acetate per min at ph 8.0 At 37 °c. Physical form: lyophilized powder containing tris buffer salts general description: molecular weight: 280 kda isoelectric point: 5.5 Extinction coefficient: e1% = 18.0 (280 Nm)acetylcholinesterase from electrophorus electricus is a tetramer composed of 4 equal subunits of 70 kda each. Each subunit contains one active site. The enzyme is a glycoprotein containing hexosamines.Typetype v-sformlyophilized powdercompositionprotein, =50% biuretpl~5.3Solubilitytris buffer: soluble1 mg/ml (0.02 M tris buffer, ph 7.5)Storage temp.-20°C