A431 Egf Cell Lysate, Cell Or Tissue Lysates Are A Useful Control For The Western Blotting Application, Each

Details:

A source that can be rich in endogenously expressed proteins, this lysate is convenient to use and provided in a denatured format allowing for immediate loading onto an sds-page gel. A431 cells (human epithelial carcinoma; atcc crl-1555) were grown to 80-90% confluency, serum starved overnight, then treated with 100ng/ml egf for 5 minutes at 37°c.Storage buffer: sds-page buffer (62mm tris ph 6.8, 2% Sds, 0.9% B-mercaptoethanol, 0.003% Bromophenol blue, 5% glycerol)western blot