Human Il-6 ELISA Kit Ii, Quantitative Determination Of Human Il-6 In Serum, Plasma, And Cell Culture Supernatant, Each

Details:

Il-6 specific monoclonal antibody binds to immobilized antibody coated on 96-wells plate. Wells are washed and streptavidinhorseradish peroxidase conjugate mixed with biotinylated anti-human il-6 antibody is added, producing an antibody-antigen-antibody “sandwich”. The wells are again washed and tmb substrate solution is added, which produces a blue color in direct proportion to the amount of il-6 present in the initial sample. The stop solution changes the color from blue to yellow, and the microwell absorbances are read at 450nm.Solid phase sandwich elisastandard range: 4.7 - 300Pg/mlsensitivity: 4pg/ml