Bub3 Mouse, Unlabeled, Clone: 31, Bd, Mouse Monoclonal Antibody, Each

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Accurate chromosome segregation requires that all pairs of sister chromatids become appropriately attached to mitotic spindles before the onset of anaphase. Cell cycle checkpoints monitor kinetochore-microtubule interactions, so that cell cycle progression can be delayed until proper chromosome attachments are formed. In yeast, bub1-3 genes are required for proper mitotic delay in response to unattached kinetochores. In mammals, the homologues to yeast bub1 and bub3 form a complex that binds kinetochores and has protein kinase activity. Bub3 contains four wd repeats, three in the n-terminus and one in the c-terminus, and a central bub1-binding domain. During prophase and prometaphase, bub3 localizes to the kinetochore before attachment to microtubules. In addition, taxol-induced formation of lagging chromosomes due to a delay of cell cycle progression increases the level of bub3 co-localized with kinetochores, while correctly aligned chromosomes found in metaphase do not exhibit this co-localization. Thus, bub3, in association with bub1, may be important for sensing kinetochore attachment to microtubules during the prometaphase to metaphase transition.Host species: mouseclone: 31isotype: igg1species reactivity [for features main]: humanimmunogen: human bub3 aa. 4-16Bioimaging, immunofluorescence, western blotting