Alanine Transaminase Colorimetric Activity Assay Kit, 96 Wells, Each

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Alt is a homodimeric cytoplasmic pyridoxal phosphate-dependent enzyme involved in cellular nitrogen metabolism, amino acid metabolism, and liver gluconeogenesis. Cayman’s alanine transaminase assay provides a convenient method of detecting alt activity in serum, plasma, tissue samples, and cell lysates. Measurement of the alt activity is carried out by monitoring the rate of nadh oxidation in a coupled reaction system employing ldh. The oxidation of nadh to nad is accompanied by a decrease in absorbance at 340 nm.