Cd138 Rat Anti-Mouse, Apc-R700, Clone: 281-2, Bd, Rat Monoclonal Antibody, Each

Details:

The 281-2 monoclonal antibody specifically binds to the core protein of cd138 (syndecan-1), a cell-surface, integral membrane heparan sulfate- and chondroitin sulfate-containing proteoglycan that binds to interstitial extracellular matrix molecules. Syndecan-1 is predominantly expressed on epithelial cells, where its expression correlates with normal epithelial organization. It is also expressed on b lymphocytes at specific stages during their differentiation: precursor b cells in the bone marrow and antibody-secreting cells, including plasma cells, but not mature peripheral b cells. It is thus implicated in mediating b cell-matrix interactions. Cd138 expression is also regulated during embryonic development, and the molecule shows a tissue- specific structural polymorphism resulting from different post-translational modifications. The 281-2 antibody may be used to detect the differently glycosylated forms, because it reacts with the core protein. Furthermore, the mab detects the syndecan-1 ectodomain which is cleaved from cell surfaces by a metalloproteinase. This antibody was conjugated to bd horizon apc-r700, which has been developed exclusively by bd biosciences as a better alternative to alexa fluor™ 700. Apc-r700 excites and emits at similar wavelengths to alexa fluor™ 700 yet exhibits significantly improved brightness. This dye can be excited by the red laser and detected with the same filter set as alexa fluor™ (eg, 730/45-nm filter).