Cd14 Mouse Anti-Human, Brilliant Violet 650, Clone: M5e2, Bd, Mouse Monoclonal Antibody, Each

Details:

The m5e2 monoclonal antibody specifically binds to cd14, a 53–55 kda glycosylphosphatidylinositol (gpi)-anchored single chain glycoprotein expressed at high levels on monocytes. Additionally, the anti-cd14 antibody reacts with interfollicular macrophages, reticular dendritic cells, and some langerhans cells. Cd14 has been identified as a high affinity cell-surface receptor for complexes of lipopolysaccharide (lps) and serum lps-binding protein, lpb. The antibody was conjugated to bd horizon™ bv650 which is part of the bd horizon™ brilliant violet™ family of dyes. This dye is a tandem fluorochrome of bd horizon™ bv421 with an ex max of 405-nm and an acceptor dye with an em max at 650-nm. Bd horizon™ bv650 can be excited by the violet laser and detected in a filter used to detect apc-like dyes (eg, 660/20-nm filter). Due to the excitation and emission characteristics of the acceptor dye, there will bespillover into the apc and alexa fluor™ 700 detectors. However, the spillover can be corrected through compensation as with any other dye combination.