Cd19 Mouse Anti-Human, Brilliant Violet 605, Clone: Sj25c1, Bd, Mouse Monoclonal Antibody, Each

Details:

The sj25c1 monoclonal antibody specifically binds to cd19, a b lymphocyte-lineage differentiation antigen. Cd19, a 90-kda transmembrance glycoprotein, is a member of the immunoglobulin superfamily and is expressed throughout b-lymphocyte development from the pro-b cell through the mature b-cell stages. Terminally differentiated plasma cells do not express cd19. On the surface of mature b cells, the cd19 molecule associates with cd21 (cr-2) and cd81 (tapa-1), and this multimolecular complex synergizes with surface immunoglobulin to promote cellular activation. Studies with cd19-deficient mice have suggested that the level of cd19 expression affects the generation and maturation of b cells in the bone marrow and periphery. B-1 lineage b cells, also known as cd5+ b cells, are drastically reduced or absent in cd19-deficient mice. Increased levels of cd19 expression correlate with increased frequencies of peritonal and splenic b-1 cells and reduced numbers of conventional b lymphocytes in the periphery. Cd19 participates in b-lymphocyte development, b-cell activation, maturation of memory b cells and regulation of tolerance. Cd19 has also been detected on peritoneal mast cells, co-localized with cd21/cd35, and it is proposed to play a role in complement-mediated mast-cell activation. This antibody is conjugated to bd horizon bv605 which is part of the bd horizon brilliant™ violet family of dyes. With an ex max of 407-nm and em max of 602-nm, bd horizon bv605 can be excited by a violet laser and detected with a standard 610/20-nm filter set. Bd horizon bv605 is a tandem fluorochrome of bd horizon bv421 and an acceptor dye with an em max at 605-nm. Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the pe and bd horizon pe-cf594 detectors off the green or yellow-green lasers. Bd horizon bv605 conjugates are very bright, often exhibiting brightness equivalent to pe conjugates and can be used as a third color off of the violet laser.