Cd19 Mouse Anti-Human, Brilliant Violet 711, Clone: Sj25c1, Bd, Mouse Monoclonal Antibody, Each

Details:

The sj25c1 monoclonal antibody specifically binds to cd19, a b lymphocyte-lineage differentiation antigen. Cd19, a 90-kda transmembrance glycoprotein, is a member of the immunoglobulin superfamily and is expressed throughout b-lymphocyte development from the pro-b cell through the mature b-cell stages. Terminally differentiated plasma cells do not express cd19. On the surface of mature b cells, the cd19 molecule associates with cd21 (cr-2) and cd81 (tapa-1), and this multimolecular complex synergizes with surface immunoglobulin to promote cellular activation. Studies with cd19-deficient mice have suggested that the level of cd19 expression affects the generation and maturation of b cells in the bone marrow and periphery. B-1 lineage b cells, also known as cd5+ b cells, are drastically reduced or absent in cd19-deficient mice. Increased levels of cd19 expression correlate with increased frequencies of peritonal and splenic b-1 cells and reduced numbers of conventional b lymphocytes in the periphery. Cd19 participates in b-lymphocyte development, b-cell activation, maturation of memory b cells and regulation of tolerance. Cd19 has also been detected on peritoneal mast cells, co-localized with cd21/cd35, and it is proposed to play a role in complement-mediated mast-cell activation. The antibody was conjugated to bd horizon™ bv711 which is part of the bd horizon™ brilliant violet™ family of dyes. This dye is a tandem fluorochrome of bd horizon™ bv421 with an ex max of 405-nm and an acceptor dye with an em max at 711-nm. Bd horizon™ bv711 can be excited by the violet laser and detected in a filter used to detect cy™5.5 / Alexa fluor™ 700-like dyes (eg, 712/20-nm filter). Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the alexa fluor™ 700 and percp-cy™5.5 Detectors. However, the spillover can be corrected through compensation as with any other dye combination.