Cd19 Rat Anti-Mouse, Pe-Cf594, Clone: 1d3, Bd, Rat Monoclonal Antibody, Each

Details:

The 1d3 antibody reacts with cd19, a b lymphocyte-lineage differentiation antigen. Cd19, a 95-kda transmembrance glycoprotein, is a member of the immunoglobulin superfamily and is expressed throughout b-lymphocyte development from the pro-b cell throughthe mature b-cell stages. Terminally differentiated plasma cells do not express cd19. On the surface of mature b cells, the cd19 molecule associates with cd21 (cr-2) and cd81 (tapa-1), and this multimolecular complex synergizes with surface immunoglobulin to promote cellular activation. Studies with cd19-deficient mice have suggested that the level of cd19 expression affects the generation and maturation of b cells in the bone marrow and periphery. B-1 lineage b cells, also known as cd5 b cells, are drastically reduced or absent in cd19-deficient mice. Increased levels of cd19 expression correlate with increased frequencies of peritonal and splenic b-1 cells and reduced numbers of conventional b lymphocytes in the periphery. Cd19 participates in b-lymphocyte development, b-cell activation, maturation of memory b cells and regulation of tolerance. Cd19 has also been detected on peritoneal mast cells, co-localized with cd21/cd35, and it is proposed to play a role in complement-mediated mast-cell activation. This antibody is conjugated to bd horizon™ pe-cf594, which has been developed exclusively by bd biosciences as a better alternative to pe-texas red. Pe-cf594 excites and emits at similar wavelengths to pe-texas red yet exhibits improved brightness and spectral characteristics. Due to pe having maximal absorption peaks at 496nm and 564nm, pe-cf594 can be excited by the blue (488-nm), green (532-nm) and yellow-green (561-nm) lasers and can be detected with the same filter set as pe-texas red (eg 610/20-nm filter).