Cd25 Mouse Anti-Human, Apc-R700, Clone: 2a3, Bd, Mouse Monoclonal Antibody, Each

Details:

The 2a3 monoclonal antibody specifically binds to human cd25, the low-affinity alpha subunit of the interleukin-2 receptor (il- 2rα). Cd25 associates with cd122 (il-2rβ chain) and cd132 (common γchain or γc) to form the high-affinity signal-transducing il -2r complex. Cd25 is expressed by subsets of thymocytes and peripheral blood lymphocytes including cd4 cd25 regulatory t cells and memory t cells. Cd25 antigen density increases on activated t cells including phytohemagglutinin (pha)-, concanavalin a (con a)-, and cd3-activated t lymphocytes. High levels of cd25 can be expressed by t lymphocytes from mixed lymphocyte cultures and by human t-lymphocyte leukemia virus (htlv)-infected t-lymphocyte leukemia lines, for example, hut-102. Cd25 can also be expressed by activated b cells and macrophages. Recombinant il-2 blocks the binding of the 2a3 antibody to pha-activated t lymphocytes. This antibody was conjugated to bd horizon apc-r700, which has been developed exclusively by bd biosciences as a better alternative to alexa fluor™ 700. Apc-r700 excites and emits at similar wavelengths to alexa fluor™ 700 yet exhibits significantly improved brightness. This dye can be excited by the red laser and detected with the same filter set as alexa fluor™ (eg, 730/45-nm filter).