Cd25 Rat Anti-Mouse, Pe-Cf594, Clone: Pc61, Bd, Rat Monoclonal Antibody, Each

Details:

The pc61 antibody reacts with cd25, the low-affinity il-2 receptor α chain (il-2rα, p55) expressed on activated t and b lymphocytes from all mouse strains tested. Il-2rα by itself is not a signaling receptor. However, it can combine with il-2 receptor β (cd122) and γc (cd132) chains to form high-affinity, signaling receptor complexes for il-2. Resting t and b lymphocytes and resting and activated nk cells do not express il-2rα. Cd25 is transiently expressed at a low level during normal b-cell development in the bone marrow on the cd45r/b220low tdt- sig- pre-b/pre-b-ii and cd45r/b220low tdt- sigm+ sigd- immature b stages, but not on the cd45r/b220low tdt+ sig- pro-b/pre-b-i stage nor on cd45r/b220high tdt- sigm+ sigd+ mature b cells. It is expressed at a higher level during a very early stage of t-cell development in fetal and adult thymus. Peripheral cd25+cd4+ lymphocytes called regulatory t (treg) cells are involved in the maintenance of self-tolerance. It has also been reported that dendritic cells express cd25, recognized by mab 7d4 (cat. No. 553068). The pc61 antibody recognizes an epitope of cd25 which is distinct from the il-2 binding site and from those recognized by mabs 3c7 (cat. No. 557364) And 7d4 (cat. No. 553068). It blocks binding of il-2 to cd25, presumably by inducing a conformational change in cd25. This antibody is conjugated to bd horizon™ pe-cf594, which has been developed exclusively by bd biosciences as a better alternative to pe-texas red™. Pe-cf594 excites and emits at similar wavelengths to pe-texas red™ yet exhibits improved brightness and spectral characteristics. Due to pe having maximal absorption peaks at 496nm and 564nm, pe-cf594 can be excited by the blue (488-nm), green (532-nm) and yellow-green (561-nm) lasers and can be detected with the same filter set as pe-texas red™ (eg 610/20-nm filter).