Cd252 Rat Anti-Mouse, Bb515, Clone: Rm134l, Bd, Rat Monoclonal Antibody, Each

Details:

The rm134l antibody reacts with cd252 (ox-40 ligand, ox-40l), a member of the ngf/tnf superfamily which is present on antigen-presenting cells and activated b lymphocytes. Ox-40l interacts with ox-40 antigen (cd134) found predominantly on activated t cells. This ligand-receptor pair is grouped with pairs such as cd40-cd40l and cd80- or cd86-cd28, which contribute significantly to b-cell/t-cell interaction during the immune response. The ox-40l-ox-40 interaction is reciprocally costimulatory in that both t cells and b cells are activated in cross-linking. Stimulation via ox-40 antigen increases the proliferative and il-2 production responses of activated t cells, while stimulation via ox-40l enhances proliferation and ig secretion by activated b cells. The rm134l mab stains b cells activated for four days with anti-igm plus anti-cd40 (hm40-3, cat. No. 553721) Antibodies. An increased binding of ox-40-ig fusion protein to mouse splenic b cells was observed when b cells were treated with lps, suggesting that ox-40l expression is augmented in lps-activated splenic b cells when compared to resting cells, but this observation could not be confirmed with the rm134l mab. In addition, other studies with ox-40-ig fusion protein detected ox-40l on cd4 and cd8 activated splenic t cells, but ox-40l was not detected on t cells with the rm134l mab. Similar results have been reported by others. The rm134l mab inhibits the binding of ox-40-ig fusion protein to ox-40l transfectants and blocks the costimulatory activity of ox-40l