Cd3 Mouse Anti-Human, Brilliant Violet 650, Clone: Ucht1, Bd, Mouse Monoclonal Antibody, Each

Details:

The ucht1 monoclonal antibody specifically binds to the human cd3 ε -chain, a 20-kda subunit of the cd3/t cell antigen receptor complex. Cd3 ε is expressed on 70-80% of normal human peripheral blood lymphocytes and 60-85% of thymocytes. Studies from the hlda workshop show that this antibody is mitogenic for cd3 ε -positive cells when used in conjunction with costimulatory agents such as pokeweed mitogen or anti-cd28 antibody. Cd3 plays a central role in signal transduction during antigen recognition. The ucht1 antibody stains both surface and intracellular cd3 ε unlike the other cd3 clone, hit3a, that stains only extracellular cd3 ε . The antibody was conjugated to bd horizon™ bv650 which is part of the bd horizon™ brilliant violet™ family of dyes. This dye is a tandem fluorochrome of bd horizon™ bv421 with an ex max of 405-nm and an acceptor dye with an em max at 650-nm. Bd horizon™ bv650 can be excited by the violet laser and detected in a filter used to detect apc-like dyes (eg, 660/20-nm filter). Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the apc and alexa fluor™ 700 detectors. However, the spillover can be corrected through compensation as with any other dye combination.