Cd45.1 Mouse Anti-Mouse, Brilliant Violet 510, Clone: A20, Bd, Mouse Monoclonal Antibody, Each

Details:

The a20 monoclonal antibody specifically binds to cd45 (leukocyte common antigen) on all leukocytes of mouse strains expressing the cd45.1 Alloantigen (eg, riii, sjl/j, sts/a, da). This alloantigen was originally named ly-5.2, And this was the designation at the time that the antibody was characterized. The designation was later changed from ly-5.2 To ly-5.1 To conform with the convention that the .2 Alloantigen designations be assigned to the c57bl/6 strain. Mab a20 has been reported not to react with leukocytes of most other mouse strains, which express the cd45.2 Alloantigen. Cd45 is a member of the protein tyrosine phosphatase (ptp) family: its intracellular (cooh-terminal) region contains two ptp catalytic domains, and the extracellular region is highly variable due to alternative splicing of exons 4, 5, and 6 (designated a, b, and c, respectively), plus differing levels of glycosylation. The cd45 isoforms detected in the mouse are cell type-, maturation-, and activation state-specific. The cd45 isoforms play complex roles in t-cell and b-cell antigen receptor signal transduction. The a20 antibody has been reported to inhibit some responses of b cells, from mice expressing the cd45.1 Alloantigen, to antigens and lps. The antibody was conjugated to bd horizon bv510 which is part of the bd horizon brilliant™ violet family of dyes. With an ex max of 405-nm and em max at 510-nm, bd horizon bv510 can be excited by the violet laser and detected in the bd horizon v500 (525/50-nm) filter set. Bd horizon bv510 conjugates are useful for the detection of dim markers off the violet laser.