$ 564.98
Details:
The ra3-6b2 antibody reacts with an epitope on the extracellular domain of cd45 glycoprotein which is dependent upon the expression of exon a and specific carbohydrate residues. It is expressed on b lyphocytes at all stages from pro-b through mature and activated b cell, but it is decreased on plasma cells and a subset of memory b cells. Levels of expression of cd45r/b220 on the b-cell lineage appear to be developmentally regulated. It is also found on the abnormal t cells involved in the lymphadenopathy of lpr/lpr and gld/gld mutant mice, on lytically active subsets of lymphokine-activated killer cells (nk cells and non-mhc-restricted ctl), on apoptotic t lymphocytes of mice injected with bacterial superantigen, on a population of nk-cell precursors in the bone marrow, and on b-lymphocyte, t-lymphocyte, and macrophage progenitors in fetal liver. The cd45r/b220 antigen is not on hematopoietic stem cells, naive t lymphocytes, or mhc-restricted ctl. Cd45 is a member of the protein tyrosine phosphatase (ptp) family: its intracellular (cooh-terminal) region contains two ptp catalytic domains, and the extracellular region is highly variable due to alternative splicing of exons 4, 5, and 6 (designated a, b, and c, respectively), plus differing levels of glycosylation. The cd45 isoforms detected in the mouse are cell type-, maturation, and activation state-specific. The cd45 isoforms play complex roles in t-cell and b-cell antigen receptor signal transduction. Cd45r/b220 is commonly used as a pan b-cell marker; however, cd19 expression, detected by mab 1d3, is reported to be more restricted to the b-cell lineage. Mab ra3-6b2 has been reported to enhance isotype switching during in vitro b-cell responses and to inhibit in vivo b-cell responses. Cross-reaction of ra3-6b2 mab with activated human t lymphocytes has been observed. This antibody is routinely tested by flow cytometric analysis. Other applications were tested at bd biosciences pharmingen during antibody development only.Host species: ratclone: ra3-6b2isotype: igg2a κspecies reactivity: mouseimmunogen: mouse pre-b tumorcell separation, flow cytometry, immunohistochemistry, immunohistochemistry (frozen)
