Cd62l Rat Anti-Mouse, Unlabeled, Clone: Mel-14, Bd, Rat Monoclonal Antibody, Each

Details:

The mel-14 monoclonal antibody specifically binds to cd62l (l-selectin), a 95 kda (on neutrophils) or 74 kda (on lymphocytes) receptor with lectin-like and epidermal growth factor-like domains. In the mouse, l-selectin is detected on most thymocytes, with the highest levels of expression on an immunocompetent subset and a population of dividing progenitor cells, and on peripheral leukocytes, including subsets of b and t lymphocytes, neutrophils, monocytes, and eosinophils. This member of the selectin adhesion molecule family appears to be required for lymphocyte homing to peripheral lymph nodes and to contribute to neutrophil emigration at inflammatory sites. L-selectin is rapidly shed from lymphocytes and neutrophils upon cellular activation; metalloproteinases may mediate the release of cd62l ectodomains from the cell surface. The level of cd62l expression, along with other markers, distinguishes naive, effector, and memory t cells. L-selectin binds to sialytaed oligosaccharide determinants on high endothelial venules (hev) in peripheral lymph nodes. In vitro studies have demonstrated that cd34, glycam-1, and madcam-1, all recognized by mab meca-79 (anti-mouse pnad carbohydrate epitope, cat. No. 553863), May be ligands for cd62l. Mel-14 mab blocks in vitro binding of lymphocytes to peripheral lymph node hev and inhibits in vivo lymphocyte extravasation into peripheral lymph nodes and late stages of leukocyte rolling.