Cd8 Mouse Anti-Human, Apc-R700, Clone: Sk1, Bd, Mouse Monoclonal Antibody, Each

Details:

The sk1 monoclonal antibody specifically binds to cd8 alpha (cd8α). Cd8α is a type i transmembrane glycoprotein and a member of the immunoglobulin superfamily. Cd8α is expressed by the majority of thymocytes, by subpopulations of αβ t cells and γδ t cells and by some nk cells. Cell surface cd8α is expressed either as a disulfide-linked homodimer (cd8αα) or as a heterodimer (cd8αβ) when disulfide-bonded to a cd8 beta chain (cd8β). Cd8-positive αβ t cells coexpress both cd8αα homodimers and cd8αβ heterodimers whereas some γδ t cells and nk cells express cd8αα homodimers. Cd8 plays important roles in t cell activation and selection. The extracellular igsf domain of cd8α binds to a non-polymorphic determinant on hla class i molecules (α3 domain) and enables cd8 to function as a co-receptor with mhc class i-restricted tcr during t cell recognition of antigen. The cytoplasmic domain of cd8α associates with lck, a src family protein tyrosine kinase that is involved in intracellular signaling. This antibody was conjugated to bd horizon apc-r700, which has been developed exclusively by bd biosciences as a better alternative to alexa fluor™ 700. Apc-r700 excites and emits at similar wavelengths to alexa fluor™ 700 yet exhibits significantly improved brightness. This dye can be excited by the red laser and detected with the same filter set as alexa fluor™ (eg, 730/45-nm filter).