Cd8 Mouse Anti-Human, Brilliant Violet 421, Clone: Rpa-T8, Bd, Mouse Monoclonal Antibody, Each

Details:

The rpa-t8 monoclonal antibody specifically binds to cd8 alpha (cd8α). Cd8α is a type i transmembrane glycoprotein and a member of the immunoglobulin superfamily. Cd8α is expressed by the majority of thymocytes, by subpopulations of αβ t cells and γδ t cells and by some nk cells. Cell surface cd8α is expressed either as a disulfide-linked homodimer (cd8αα) or as a heterodimer (cd8αβ) when disulfide-bonded to a cd8 beta chain (cd8β). Cd8-positive αβ t cells coexpress both cd8αα homodimers and cd8αβ heterodimers whereas some γδ t cells and nk cells express cd8αα homodimers. Cd8 plays important roles in t cell activation and selection. The extracellular igsf domain of cd8α binds to a non-polymorphic determinant on hla class i molecules (α3 domain) and enables cd8 to function as a co-receptor with mhc class i-restricted tcr during t cell recognition of antigen. The cytoplasmic domain of cd8α associates with lck, a src family protein tyrosine kinase that is involved in intracellular signaling. The rpa-t8 and hit8a (cat. No. 555337) Monoclonal antibodies are not cross-blocking. The antibody was conjugated to brilliant violet 421 and has been developed in collaboration with sirigen. With an ex max of 407-nm and em max at 421-nm, brilliant violet 421 can be excited by the violet laser and detected in the standard pacific blue filter set (eg, 450/50-nm filter). Brilliant violet 421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to pacific blue conjugates.Host species: mouseclone: rpa-t8isotype: igg1, κspecies reactivity: humanflow cytometry