Cd8 Mouse Anti-Human, Brilliant Violet 605, Clone: Sk1, Bd, Mouse Monoclonal Antibody, Each

Details:

The sk1 monoclonal antibody specifically binds to cd8 alpha (cd8α). Cd8α is a type i transmembrane glycoprotein and a member of the immunoglobulin superfamily. Cd8α is expressed by the majority of thymocytes, by subpopulations of αβ t cells and γδ t cells and by some nk cells. Cell surface cd8α is expressed either as a disulfide-linked homodimer (cd8αα) or as a heterodimer (cd8αβ) when disulfide-bonded to a cd8 beta chain (cd8β). Cd8-positive αβ t cells coexpress both cd8αα homodimers and cd8αβ heterodimers whereas some γδ t cells and nk cells express cd8αα homodimers. Cd8 plays important roles in t cell activation and selection. The extracellular igsf domain of cd8α binds to a non-polymorphic determinant on hla class i molecules (α3 domain) and enables cd8 to function as a co-receptor with mhc class i-restricted tcr during t cell recognition of antigen. The cytoplasmic domain of cd8α associates with lck, a src family protein tyrosine kinase that is involved in intracellular signaling. This antibody is conjugated to bd horizon bv605 which is part of the bd horizon brilliant™ violet family of dyes. With an ex max of 407-nm and em max of 602-nm, bd horizon bv605 can be excited by a violet laser and detected with a standard 610/20-nm filter set. Bd horizon bv605 is a tandem fluorochrome of bd horizon bv421 and an acceptor dye with an em max at 605-nm. Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the pe and bd horizon pe-cf594 detectors off the green or yellow-green lasers. Bd horizon bv605 conjugates are very bright, often exhibiting brightness equivalent to pe conjugates and can be used as a third color off of the violet laser.