Cd8 Mouse Anti-Human, Brilliant Violet 650, Clone: Sk1, Bd, Mouse Monoclonal Antibody, Each

Details:

The sk1 monoclonal antibody specifically binds to cd8 alpha (cd8α). Cd8α is a type i transmembrane glycoprotein and a member of the immunoglobulin superfamily. Cd8α is expressed by the majority of thymocytes, by subpopulations of αβ t cells and γδ t cells and by some nk cells. Cell surface cd8α is expressed either as a disulfide-linked homodimer (cd8αα) or as a heterodimer (cd8αβ) when disulfide-bonded to a cd8 beta chain (cd8β). Cd8-positive αβ t cells coexpress both cd8αα homodimers and cd8αβ heterodimers whereas some γδ t cells and nk cells express cd8αα homodimers. Cd8 plays important roles in t cell activation and selection. The extracellular igsf domain of cd8α binds to a non-polymorphic determinant on hla class i molecules (α3 domain) and enables cd8 to function as a co-receptor with mhc class i-restricted tcr during t cell recognition of antigen. The cytoplasmic domain of cd8α associates with lck, a src family protein tyrosine kinase that is involved in intracellular signaling. The antibody was conjugated to bd horizon bv650 which is part of the bd horizon brilliant™ violet family of dyes. This dye is a tandem fluorochrome of bd horizon bv421 with an ex max of 405-nm and an acceptor dye with an em max at 650-nm. Bd horizon bv650 can be excited by the violet laser and detected in a filter used to detect apc-like dyes (eg, 660/20-nm filter). Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the apc and alexa fluor™ 700 detectors. However, the spillover can be corrected through compensation as with any other dye combination.