Cd86 Mouse Anti-Human, Brilliant Violet 650, Clone: 2331 (Fun-1), Bd, Mouse Monoclonal Antibody, Each

Details:

The 2331 (fun-1) monoclonal antibody specifically recognizes a 75 kda transmembrane cell surface protein, cd86 (b70/b7-2), expressed primarily on monocytes, dendritic cells and activated b cells. Competitive binding assays demonstrate that, while both 2331 (fun-1) and it2.2 (Anti-cd86, cat. No. 555663) Antibodies specifically recognize the same molecule, they react with different epitopes. Cd86 is the second ligand for cd28 and ctla-4 and may play an important role in co-stimulation of t cells in primary immune response. The 2331 (fun-1) antibody blocks the costimulatory activity of cd86 when tested in functional studies. The antibody was conjugated to bd horizon™ bv650 which is part of the bd horizon™ brilliant violet™ family of dyes. This dye is a tandem fluorochrome of bd horizon™ bv421 with an ex max of 405-nm and an acceptor dye with an em max at 650-nm. Bd horizon™ bv650 can be excited by the violet laser and detected in a filter used to detect apc-like dyes (eg, 660/20-nm filter). Due to the excitation and emission characteristics of the acceptor dye, there will bespillover into the apc and alexa fluor™ 700 detectors. However, the spillover can be corrected through compensation as with any other dye combination.