Cd8a Rat Anti-Mouse, Bb515, Clone: 53-6.7, Bd, Rat Monoclonal Antibody, Each

Details:

The 53-6.7 Antibody monoclonal antibody specifically binds to the 38 kda α and 34 kda α chains of the cd8 differentiation antigen (ly-2 or lyt-2) of all mouse strains tested. The cd8 α and α chains (cd8a) form heterodimers with the cd8 β chain (cd8b, ly-3, or lyt-3) on the surface of most thymocytes. A subpopulation of mature t lymphocytes (i.E., Mhc class i-restricted t cells, including most t suppressor/cytotoxic cells) expresses almost exclusively the cd8 αβ heterodimer (the αα chains arise from alternatively spliced messengers of a single cd8a gene. The longer α form associates with p56 [lck] via a cxcp motif in its cytoplasmic domain, which it shares with cd4, but not with cd8b. The truncated α chain is unable to associate with p56 [lck], and it may function to attenuate the cd8-mediated costimulatory signal during intrathymic t-cell maturation. In vivo and in vitro treatment with 53-6.7 Mab has reportedly been effective at depleting cd8+ peripheral t lymphocytes. The 53-6.7 Antibody has also been reported to cross-reaact with cd8 α- and α-like polypeptides on subsets of thymic and peripheral lymphocytes in the egyptian toad, bufo regularis.