Cdk1/Cdc2 (Py15) Mouse, Unlabeled, Clone: 44, Bd, Mouse Monoclonal Antibody, Each

Details:

Progression of the mammalian cell cycle is regulated by phosphorylation of many key proteins. Several classes of cyclins (a-e) act as regulatory subunits for cyclin-dependent kinases (cdks). Cdk1/cdc2 (p34 [cdc2]) is the catalytic subunit of the maturation promoting factor (mpf), which includes the regulatory subunit cyclin b. During late s and g2 phase, cyclin b synthesis increases, allowing it to bind cdc2. This begins the transition into m-phase of the mammalian cell cycle by initiating a series of phosphorylation and dephosphorylation events that lead to activation of the cdc2/cyclin b complex. After binding to cyclin b, cdc2 is phosphorylated on thr-14, by myt1, and tyr-15, by wee1 or mik1, yielding an inactive pre-mpf complex during g2 phase. Phosphorylation of cdc2 on thr-161 is performed by a cdk7/cyclin h complex and is necessary for activation of the cdc2 complex. Dephosphorylation of thr-14 and tyr-15 by cdc25 occurs at the end of g2 phase and completes activation of the cdc2/cyclin b complex and facilitates entry into mitosis. During mitosis, cyclin b is targeted for degradation and cdc2 becomes inactive again.Immunofluorescence, western blotting