Cdk1 Mouse, Unlabeled, Clone: 1, Bd, Mouse Monoclonal Antibody, Each

Details:

Cdk1, also known as p34 [cdc2], is a ubiquitously expressed serine/threonine protein kinase. Cdk1/cdc2 has been identified as the catalytic subunit of the maturation-promoting factor (mpf), while cyclin b acts as the regulatory subunit. The binding of these two subunits is critical to the transition into m-phase of the mammalian cell cycle, and this factor's role is regulated by a series of phosphorylations and dephosphorylations. After binding to cyclin b, cdk1/cdc2 is phosphorylated on thr-14, by myt1, and tyr-15, by wee1 or mik1, yielding an inactive pre-mpf complex. Phosphorylation of cdk1/cdc2 on thr-161 is performed by a cdk7/cyclin h complex and is necessary for activation of the cdc2 complex. Dephosphorylation of thr-14 and tyr-15 by cdc25 occurs at the prophase/metaphase transition and completes activation of the cdc2/cyclin b complex, initiating the cell's entry into mitosis.