Dehydroepiandrosterone Sulfate (Dhea-S) Competitive Elisa Kit, Competitive Elisa, Each

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The dehydroepiandrosterone sulfate (dhea-s) elisa quantitates dhea-se in serum,plasma,urine,saliva,dried fecal extracts or cell culture medium.Principle of the methodthe dehydroepiandrosterone sulfate (dhea-s) competitive elisa research-use-only kit uses a specifically generated antibody to measure dehydroepiandrosterone sulfate (dhea-s) in serum,plasma,urine,and saliva samples,and in fecal extracts. The kit will also quantitatively measure dhea-s present in tissue culture media samples. A dhea-s standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture sheep antibodies. A dhea-s-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a polyclonal antibody to dhea-s to each well. After a 2-hour incubation the plate is washed and substrate is added. The substrate reacts with the bound dhea-s-peroxidase conjugate. After a short incubation,the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring at 450 nm.Rigorous validationeach manufactured lot of this elisa kit is quality tested for criteria such as sensitivity,specificity,precision,and lot-to-lot consistency. See manual for more information on validation.Dehydroepiandrosterone sulfate (dhea-s) is the major c19 steroid secreted by the adrenal cortex and a precursor in testosterone and estrogen biosynthesis. Dhea-s is identical across all species and so this kit should measure dhea-s from all sources. It is produced by the addition of a sulfate group to dehydroepiandrosterone (dhea), catalyzed by the sulfotransferase enzymes, sult1a1 and sult1e1, which also produce estrone sulfate from estrone. Dhea sulfate can also be back-converted to dhea through the action of steroid sulfatase.