Dipeptidyl Peptidase Iv Human Recombinant, Expressed In Baculovirus Infected Sf9 Cells, Pkg Of >=1.0 Units/Vial, >=10 Units/mg Protein, 1 Vial, Each

Details:

Specifications synonyms: cd26; dppiv; dipeptidyl aminopeptidase iv; glycoprotein gp110mdl no.: Mfcd00166456purity: >=10 units/mg proteinstorage: -20cenzyme commission (ec) number: 3.4.14.5  ( Brenda | iubmb )unspsc code: 12352204general description: c-terminal histidine-tagged. Soluble form (residues 29-766) mw 105 kdaapplication: the enzyme from sigma has been used to study the lc-ms (liquid chromatography-mass spectrometry) based assay method for dpp-iv inhibitor screening and substrate discovery.Application: human dipeptidyl peptidase iv has been used to study interactive hemodynamic effects of its inhibition and angiotensin-converting enzyme inhibition in humans. Human dipeptidyl peptidase iv has also been used in a study that informed the understanding of hymenoptera venom allergies.Biochem/physiol actions: native dppiv is a ubiquitous type ii transmembrane glycoprotein and a serine protease of the s9 prolyl-oligopeptidase family. In vivo, it is synthesized with a signal peptide, which functions as the membrane anchoring domain. There is an 88% sequence homology between the human and porcine kidney enzymes. Both exist as homodimers with a subunit molecular weight of ~30 kda. The high mannose 100 kda dppiv precursor is processed in the golgi to yield a 124 kda heavily n-and o-linked mature glycoprotein. It is then sorted to the apical membrane through the concerted action of both n- and o-linked glycans and its association with lipid microdomains. The porcine enzyme contains 18.3% Carbohydrates, which the glycan composition is 0.9% Fucose, 3.4% Mannose, 5.1% Galactose, 8.2% Glucosamine, and 0.7% Sialic acid. Dppiv is highly expressed on endothelial cells, epithelial cells, and lymphocytes. It is also present in plasma in its soluble form.Biochem/physiol actions: dppiv has a post-proline dipeptidyl aminopeptidase activity that hydrolyzes n-terminal dipeptides from the unsubstituted n-terminus of peptides with the sequence of x-pro-z and x-ala-z. The optimum ph is found to be 7.4-8.7. Dppiv is involved in the regulation of several important physiological processes such as immune functions, inflammation, cns, endocrine functions, bone marrow mobilization, cancer growth, cell adhesion, glucose hemostasis and sepsis/severe infection.Physical form: supplied as a solution in 10 mm tris-hcl, ph 7.6, 200 Mm nacl, 1 mm edta and 10% glycerol.Unit definition: one unit will produce 1.0 Mumole of p-nitroaniline from gly-l-pro p-nitroanilide per min in 100 mm tris-hcl at ph 7.6 At 37 c.Other notes: view more information on dipeptidyl peptidase iv at www.Sigma-aldrich.Com/enzymeexplorer.Ridadr: nonh for all modes of transportwgk germany: 1