Dna Glow Lab: Exploring Dna Structure, Kit

Details:

Intermediate–easy to perform; requires some background knowledge.In this lab, students use a fluorescent dye to investigate the conditions that influence dna structure and its transition from double helix to single strand, and vice versa. Students can test conditions that influence watson-crick base pairing between complementary dna strands, using temperature to differentiate between dna of different sequences, and establishing the melting temperature of each sample. They carry out 3 investigations exploring dna composition, concentration, the chemical milieu, and their influence on watson-crick pairing. Includes sufficient materials for a class of 32 working in 8 groups. Note: ships with perishable materials. Keep refrigerated until use.