Drai, Hc (50 U/ΜL), Drai Is Available As A Fastdigest Enzyme Which Is Used For Rapid Dna Digestion, Each

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Thermo scientific™ drai is available as a fastdigest™ enzyme which is used for rapid dna digestion.5'...T t t▵a a a...3'3'...A a a▵t t t...5'Conditions for 100% activity: 1x buffer tango™: 33mm tris-acetate (ph7.9 At 37°c), 10mm mg-acetate, 66mm k-acetate and 0.1Mg/ml bsa incubate at 37°cstorage buffer: drai is supplied in:10mm tris-hcl (ph 7.5 At 25°c), 50mm kcl, 1mm dtt, 0.1Mm edta, 0.15% Triton x-100, 0.2Mg/ml bsa and 50% (v/v) glycerolligation and recleavage: after 50-fold overdigestion with drai, more than 95% of the dna fragments can be ligated and recutmethylation effects: dam: never overlaps — no effect dcm: never overlaps — no effect cpg: never overlaps — no effect ecoki: may overlap — blocked ecobi: never overlaps — no effectdigestion of agarose-embedded dna: minimum 5units of the enzyme are required for complete digestion of 1μg of agarose-embedded lambda dna in 16hour