Dulbecco'S Modified Eagle'S Medium - High Glucose, With 4500 mg/L Glucose And Sodium Bicarbonate, Without L-Glutamine And Sodium Pyruvate, Liquid, Sterile-Filtered, Suitable For Cell Culture, Suitable For Hybridoma, 500 ml, Each

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Specifications synonym(s): dme; dmemapplication: dulbecco′s modified eagle′s medium (dmem) is a modification of basal medium eagle (bme) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/l of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of l-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/l, contributing to the name "dmem/high ".General description: this dmem-hi glucose medium is a 1x complete medium with no added factors (common modifications) such as l-alanyl-l-glutamine, hepes, or sodium pyruvate. It differs from the original dmem-hi formulation wherein pyridoxine is substituted for pyridoxal. Pyridoxal is an unstable component of media. This medium requires supplementation with l-glutamine or l-alanyl-l-glutamine.Storage: 2-8°creconstitution: supplement with 0.584 G/l l-glutamine.Empirical formula (hill notation): c6h8o