Mouse Il-5 Elisa Standard, Recombinant Protein, Each

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Il-5 is a cytokine that acts as a growth and differentiation factor for both b cells and eosinophils. Il-5 cytokine is a main regulator of eosinopoiesis, eosinophil maturation and activation. The elevated production of this cytokine is reported to be related to asthma or hypereosinophilic syndromes. The receptor of il-5 cytokine is a heterodimer, whose beta subunit is shared with the receptors for interleukine 3 (il3) and colony stimulating factor 2 (csf2/gm-csf). Il-5, together with those for interleukin 4 (il4), interleukin 13 (il13), and csf2, form a cytokine gene cluster on chromosome 5. Il-5, il4, and il13 are found to be regulated coordinately by long-range regulatory elements spread over 120 kilobases on chromosome 5q31. : Recombinant mouse il-5 is a disulfide-linked homodimer, containing two 113 amino acid peptides; these resolve as 32-34 kda bands in sds-page. Il-5 is produced by t cells and has been known as eosinophil-differentiating factor (edf), b cell growth factor ii (bcgfii), and t cell-replacing factor (trf). Il-5 induces eosinophil differentiation and promotes eosinophil survival and activation. In mice, il-5 has been shown to stimulate b cell proliferation and antibody production. Applications reported: recombinant mouse il-5 is biologically active. The intended use of the recombinant mouse il-5 single-use elisa rsg standard is as a standard for mouse il-5 ready-set-go! Elisa and mouse il-5 matched antibody pair elisa. Typically the standard is run in a series of 7 doubling dilutions from the ′top′ standard described below. Instructions for use: for ready-set-go! Elisa set (tmb substrate). Dilution: add 5ul of standard solution to 10 ml of assay diluent to prepare the top standard solution (500 pg/ml).For matched antibody pair elisa (abts or super aquablue substrate). Dilution: add 10ul of standard solution to 10 ml of assay diluent to prepare the top standard solution (1000 pg/ml). Note: these are single-use vials. Use one time and discard. Dilute as suggested above, not difficult-to-measure fractions of the suggested volumes (e.G., Not 0.5Ul in 1.0 Ml) or recovery may not be accurate. Use within 6 hours of dilution to top standard concentration. Applications tested: this cytokine standard has been calibrated, and subsequently tested by sandwich elisa using the mouse il-5 elisa pair (trfk5/trfk4). Source: insect cells infected with baculovirus: amino acids met 21-gly 133 of mouse il-5 accession # nm_010558. Purity: >98% as determined by sds-page. Storage and handling: for best recovery, quick-spin vial prior to opening. For dilution of current stock, use elisa assay diluent.