$ 1,206.90
Details:
The instantone™ elisa is specifically engineered for accurate measurement of total and phosphorylated human nfkb p65 in cell lysates. Detection of mouse and rat nfkb is expected. The instantone™ elisa kit allows for fast analysis of samples in approximately one hour. All reagents used in a traditional sandwich elisa are added in solution to a plate followed by a wash step and detection with the tmb colorimetric substrate.Nf-kappab (nuclear factor kappa b) is a nuclear transcription factor found in all cell types and is involved in cellular responses to stimuli such as stress, cytokines, free radicals, ultraviolet irradiation, and bacterial or viral antigens. Nf-kappab plays a key role in regulating the immune response to infection. Consistent with this role, incorrect regulation of nf-kappab has been linked to cancer, inflammatory and autoimmune diseases, septic shock, viral infection and improper immune development. There are five members in the nf-kappab family: nf-kappab1, nf-kappab2, rela (also named p65), relb, and c-rel. The most common form of nf-kappab is the p50/rela heterodimer, although other forms of nf-kappab dimers, such as p50/p50, p52/p52, p52/rela, p50/c-rel, c-rel/c-rel, p52/relb, and p50/relb, have also been identified in some types of cells. The primary role of nf-kappab is to maintain normal cellular functions that range from cell-to-cell communication to cell motility, cell cycle progression, and cell lineage development. The activity of nf-kappab is tightly regulated by interaction with inhibitory ikappab proteins.
