Eif-4e Mouse, Unlabeled, Clone: 87, Bd, Mouse Monoclonal Antibody, Each

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The eukaryotic translation initiation factor 4e (eif-4e) is a 25kda phosphoprotein that specifically binds to the 7-methylguanosine-containing cap of mrna. Eif-4e is the rate-limiting component for the initiation of cap-dependent translation by the eif-4e translation initiation complex. This complex promotes the unwinding of secondary structure at the 5′ untranslated region of mrna, which is necessary to expose and locate the aug-initiation codon. Phosphorylation of eif-4e on ser-209 occurs after serum treatment in cho cells, and may regulate its function. Overexpression of eif-4e can lead to increased cell proliferation, transformation, and tumorigenesis in nude mice. The overexpression of a ala-53 variant of eif-4e cannot evoke these changes, suggesting that ser-53 on eif-4e participates in the transfer of mrna to the 48s initiation complexes. In cooperation with nuclear oncogenes such as c-myc or e1a, eif-4e transforms primary cells. Other studies have demonstrated that overexpression of eif-4e causes activation of ras and leads to a transformed phenotype. Subsequent overexpression of gap then causes reversion of this phenotype. The mechanism by which eif-4e plays a role in transformation is not clear, but it is postulated that high levels of eif-4e may lead to the translation of mrnas that are normally translationally repressed.Host species: mouseclone: 87isotype: igg1species reactivity [for features main]: humanimmunogen: rabbit eif-4e aa. 1-217Immunofluorescence, immunoprecipitatio