Et-48b( ) Dna Vector, Each

Details:

The pet-48b( ) vector is designed for cloning and high-level expression of target proteins fused with the 109aa trx·tag™ thioredoxin protein that is cleavable with the human rhinovirus (hrv) 3c protease. Cloning sites are available for producing fusion proteins also containing a cleavable n- terminal his·tag™ sequence for detection and purification. The plasmid contains a strong t7lac promoter, optimized rbs, the coding sequence for the rhinovirus 3c protease cleavage site (leugluvalleupheglnglypro) for n-terminal fusion tag removal and a multiple cloning site that contains restriction enzyme sites found in many other novagen expression vectors to facilitate insert transfer. An optional c-terminal s·tag™ coding sequence is compatible with purification, detection, and quantification.