T7blue Dna (Uncut), Each

Details:

T7blue contains the puc19 backbone (including high-copy number origin of replication and lac sequences), a t7 promoter, f1 origin of replication, and modified multiple cloning region. The multiple cloning region contains an ecor v site used for t-cloning flanked by an nde i site, which allows inserts to be conveniently subcloned into the nde i site of many pet vectors.