Eps8 Mouse, Unlabeled, Clone: 15, Bd, Mouse Monoclonal Antibody, Each

Details:

The p97 [eps8] protein, a substrate for egf-r tyrosine kinase, contains an sh3 domain, but lacks a functional sh2 domain. Antibodies to eps8 recognize the 97 kda protein and a less abundant 68 kda protein. Both forms are tyrosine-phosphorylated following treatment of cells with egf. It is likely that p68 [eps8] is synthesized from an alternatively spliced mrna since two major eps8-specific mrnas are detected by northern analysis. Co-immunoprecipitation studies have demonstrated a physical association between the eps8 protein and the egf-r both in vivo and in vitro. For many egf-r substrates, this interaction is mediated through an sh2 domain of the substrate. Since eps8 lacks a well defined sh2 domain and a fusion protein containing the sh2-like region of eps8 could not bind egf-r, the mechanism of eps8-egf-r association remains unclear. Overexpression of eps8 in fibroblasts and hematopoietic cells results in an increased mitogenic response to egf, suggesting that eps8 has a role in the modulation of egf-r function.