Estrone-3-Glucuronide (E1g) Competitive Elisa Kit, Competitive Elisa, Each

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The estrone-3 glucuronide (e1g) elisa quantitates estrone-3 glucuronide in extracted serum,plasma,urine,dried fecal extracts or cell culture medium. Principle of the methodthe estrone-3-sulfate (e1s) competitive elisa research-use-only kit uses a specifically generated antibody to measure estrone-3-sulfate (e1s) in a variety of matrices,including serum,plasma,urine,and fecal samples. The kit will quantitatively measure e1s present in diluted buffer samples and tissue culture media samples. An e1s standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Standards or diluted samples are pipetted into a clear microtiter plate coated with an antibody to capture rabbit antibodies. An e1s-peroxidase conjugate is added to the standards and samples in the wells. The binding reaction is initiated by the addition of a polyclonal antibody to e1s to each well. After a 2-hour incubation the plate is washed and substrate is added. The substrate reacts with the bound e1s-peroxidase conjugate. After a short incubation,the reaction is stopped and the intensity of the generated color is detected in a microtiter plate reader capable of measuring at 450 nm.Rigorous validationeach manufactured lot of this elisa kit is quality tested for criteria such as sensitivity,specificity,precision,and lot-to-lot consistency. See manual for more information on validation.Estrone-3-glucuronide (e1g) is the principle secreted form of circulating estradiol in mammals. Estrone-3-glucuronide (e1g) is identical across all species.