Ex-Cell® 302 Serum-Free Medium For Cho Cells, Without L-Glutamine, Liquid, Sterile-Filtered, Suitable For Cell Culture, 500 ml, Each

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Specifications application: ex-cell 302 is a serum-free medium which has been specifically developed for the long-term growth of transformed chinese hamster ovary (cho) cells in suspension for the expression of antibodies or protein products. Ex-cell 302 is an appropriate medium for use with the dhfr- or glutamine synthetase, or the gs system™, because it does not contain hypoxanthine, thymidine or l-glutamine. For use with the dhfr- selection system we recommend the addition of 4 mm l-glutamine for optimal growth. Due to current regulatory concerns about the sources of raw materials, ex-cell 302 was developed using only recombinant human proteins that have molecular weights less than 10 kda. The total protein concentration found in ex-cell 302 is less than 1 mg/l. Pluronic® f68 has been added at a final concentration of 0.1% To ex-cell 302 to protect against shear damage in sparged bioreactor systems.Caution: do not freeze. Protect from light. Several components of the medium are light labile and should not be exposed to light for lengthy periods of time.Legal information: ex-cell is a registered trademark of sigma-aldrich co. Llcstorage: 2-8°cec no.: 231-791-2Empirical formula (hill notation): c63h88con14o14p