Exonuclease Iii (200 U/ΜL), Digest Double-Stranded Dna Or Dna-Rna Hybrids With Exonuclease Iii Is A 3'–>5' Exonuclease, Each

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Exonuclease iii (exoiii) exhibits four catalytic activities (see reference1). The 3'=>5' exodeoxyribonuclease activity of exoiii is specific for double-stranded dna. Exoiii degrades dsdna from blunt ends, 5'-overhangs or nicks, releases 5'-mononucleotides from the 3'-ends of dna strands and produces stretches of single-stranded dna. It is not active on 3'-overhang ends of dna that are at least four-bases long and do not carry a 3'-terminal c-residue (see reference 2) on single-stranded dna, or on phosphorothioate-linked nucleotides.Exoiii 3'-phosphatase activity removes the 3'-terminal phosphate, generating a 3'-oh group. Exoiii rnase h activity exonucleolytically degrades the rna strand in rna-dna hybrids. Expooo apurinic/apyrimidinic-endonuclease activity cleaves phosphodiester bonds at apurinic or apyrimidinic sites to produce 5'-termini that are base-free deoxyribose 5'-phosphate residues.Highlightsactive in restriction enzyme buffer