Expi293 Membranepro Expression System, Expi293 Membranepro Expression System, Each

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For researchers studying g protein–coupled receptors (gpcrs) and other membrane proteins, the expi293™ membranepro™ expression system delivers enriched, functional membrane proteins with high efficiency, scalability and reliability for a wide variety of low or high throughput applications. Features of the expi293™ membranepro™ expression system include: efficient—more than 20-fold increase in membrane protein yield compared to the adherent culture system scalable—easy scale-up without the need for multiple flasks easy to use—no enzymatic or mechanical dissociation required in addition, benefits of membranepro™ technology include: proteins are displayed on human (or other mammalian) cell membranes cellular quality control and mammalian post-translational processing helps produce functional protein proteins bud off as lipoparticles and are easily collected from the culture medium lipoparticles are enriched with expressed receptors with the less labor-intensive expi293™ membranepro™ expression system protocol, you will: 1. Clone your gene of interest using a topo™ vector 2. Transfect a susupension culture of expi293f™ cells using expifectamine™ 293 transfection reagent 3. Harvest lipoparticles 48 hrs after addition of enhancers using membranepro™ precipitation mix 4. Store lipoparticles at -80 c or proceed to downstream assays note: exi293f™ cells (a14527) and a pef6 v5-his topo™ expression kit (k9610-20) are required for use of this system and must be purchased separately when needed.