Fak (Py397) (Focal Adhesion Kinase) Mouse Anti-Human, Unlabeled, Clone: 14, Bd, Mouse Monoclonal Antibody, Each

Details:

Focal adhesion kinase (fak) is a cytoplasmic tyrosine kinase that colocalizes with integrins in focal adhesions. This cellular localization is directed by a 125 amino acid sequence at the c-terminus called the “focal adhesion targeting” sequence (fat). The binding of extracellular matrix ligands to integrins triggers autophosphorylation at tyr-397, and activation of fak through phosphorylation of tyr residues (tyr-576 and tyr577) in the kinase domain activation loop. For example, cell adhesion to a fibronectin substratum involves concurrent activation of src and phosphorylation of the fak activation loop. In addition, phosphorylation of other tyr residues (tyr-925, and tyr-861) creates binding sites for sh2 domains of intracellular signaling molecules such as src, pi3 kinase, and grb2. Fak's ability to bind numerous structural and signaling proteins via a variety of interactions is important for fak activation level, and for fak interaction with a variety of substrates localized to sites of cell adhesion. Thus, fak activity is regulated by a complex set of phosphorylation sites, and thisphospho-regulation could be important for cell motility, cell growth, cytoskeletal organization, and adhesion-dependent cell survival.Host species: mouseclone: 14isotype: igg1species reactivity [for features main]: humanimmunogen: human fak (py397) peptideimmunofluorescence, western blotting