Fastdigest Eco47i, Cut At G^Gwcc Sites With Fastdigest Avaii (Eco47i), Which Performs Best At 37°C In 5-15 Minutes Using Universal Fastdigest Buffer, Each

Details:

Lambda dna digested with avaii, 1% agarose, 35 cleavage sitesfastdigest enzymes are an advanced line of fast restriction enzymes that are all 100% active in the universal fastdigest and fastdigest green reaction buffers.The universal buffer allows rapid single-, double- or multiple dna digestion within 5-15 minutes eliminating any need for buffer change or subsequent dna clean-up steps. Dna modifying enzymes, such as klenow fragment, t4 dna ligase, alkaline phosphatases and t4 dna polymerase all have 100% activity in fastdigest buffer. Therefore, enzymes for downstream applications can be directly added to the fastdigest reaction mix.For additional convenience fastdigest green buffer includes a density reagent and two tracking dyes for direct loading of digestion reaction products on gels.Short incubation times and optimal composition of the universal fastdigest buffer eliminate star activity effects.Highlights100% activity of all fastdigest enzymes in the universal buffer100% buffer compatibility with downstream applicationscomplete digestion in 5-15 minutesdirect loading on gelsno star activity176 fastdigest enzymes available