Fastdigest Csp6i, Cut At G^Tac Sites With Fastdigest Csp6i, Which Performs Best Using Universal Fastdigest Buffer (Isoschizomers: Afai, Cviqi, Rsani), Each

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Lambda dna digested with csp6i, 1.4% Agarose, 113 cleavage sitesfastdigest enzymes are an advanced line of fast restriction enzymes that are all 100% active in the universal fastdigest and fastdigest green reaction buffers.The universal buffer allows rapid single-, double- or multiple dna digestion within 5-15 minutes eliminating any need for buffer change or subsequent dna clean-up steps. Dna modifying enzymes, such as klenow fragment, t4 dna ligase, alkaline phosphatases and t4 dna polymerase all have 100% activity in fastdigest buffer. Therefore, enzymes for downstream applications can be directly added to the fastdigest reaction mix.For additional convenience fastdigest green buffer includes a density reagent and two tracking dyes for direct loading of digestion reaction products on gels.Short incubation times and optimal composition of the universal fastdigest buffer eliminate star activity effects.Highlights100% activity of all fastdigest enzymes in the universal buffer100% buffer compatibility with downstream applicationscomplete digestion in 5-15 minutesdirect loading on gelsno star activity176 fastdigest enzymes available