Fyn Mouse, Unlabeled, Clone: 25, Bd, Mouse Monoclonal Antibody, Each

Details:

Stimulation of the t cell antigen receptor (tcr) results in protein tyrosine phosphorylation via non-receptor tyrosine kinases. The src-related p59[fyn] kinase is one such enzyme that associates with the tcr. Ligation of the tcr activates the protein kinase activity of p59[fyn] invarious human t cells. Fyn interacts with the cd3-ζ chains through its n-terminal region. In turn, fyn binds other proteins through its sh2 and sh3 domains. These proteins (p82 and p116) may serve as substrates and/or mediators of fyn activity. A 72kda fyn-related protein binds to the tcr/cd3 complex in certain t cells. This protein, known as p72[fyn-r], is a variant of the fyn kinase and contains at least four novel ser/thr phosphorylation sites. These sites may make p72[fyn-r] less susceptible to the action of phosphatases than p59[fyn]. Like p59[fyn], p72[fyn-r] is constitutively associated with its receptor. The exact relationship between these two proteins is not yet clear. However, p72[fyn-r] immunoprecipitates with various antisera raised to either the n- or c-terminus of fyn. Similar to src and yes, fyn is highly expressed in brain. The localization of fyn in mouse brain and its interaction with myelin-associated glycoprotein (mag) suggests that the fyn kinase has a role in the sensory network and in myelination during early stages of cns formation.Immunofluorescence, immunohistochemistry, immunoprecipitation, western blotting