Geneart Crispr Nuclease Vector With Cd4 Enrichment Kit, Simple, Ready-To-Use, All-In-One Expression Vector System For Crispr-Based Genome Editing, Each

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Simple, ready-to-use, all-in-one expression vector system for expression of the functional components needed for crispr-cas9 genome editing in mammalian cells with a cd4 reporter.Geneart crispr nuclease vector with cd4 enrichment kit is a vector system for expression of the functional components needed for crispr-cas9 genome editing in mammalian cells with a cd4 reporter. The cd4 reporter enables bead-based enrichment, an option for magnetic bead-based sorting/enrichment of cas9 & crispr expressing cells using dynabeads cd4 magnetic beads. Transfection efficiency can also be tracked using anti-cd4 fluorescent antibodies. The linearized geneart crispr nuclease vectors provide a rapid and efficient way to clone double-stranded oligonucleotides encoding a crrna representing a desired target into an expression cassette that allows sequence-specific targeting of the cas9 nuclease. A version with competent cells is also available (cat. No. A21177). Geneart™ crispr nuclease vector system offers: • easy-to-design genome engineering system • affordable, ready-to-use cloning vectors • enrichment for hard to transfect or difficult to modify cell for research use only. Not for use in diagnostic procedures.Certificationscoacompliancecoarecommended storagestore in freezer (-5 to -30°c)