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Specifications synonym: ß-d-glucose:oxygen 1-oxidoreductase, g.Od., Goxcas number: 9001-37-0ec number: 232-601-0enzyme commission (ec) number: 1.1.3.4Mdl number: mfcd00131182analysis note protein determined by biuretapplication glucose oxidase is widely used in the food and pharmaceutical industries1 as well as a major component of glucose biosensors.2Biochem/physiol actions glucose oxidase catalyses the oxidation of ß-d-glucose to d-glucono-ß-lactone and hydrogen peroxide, with molecular oxygen as an electron acceptor.3,4Quality may contain traces of amylase, maltase, glycogenase, invertase, and galactose oxidase.Unit definition one unit will oxidize 1.0 µMole of ß-d-glucose to d-gluconolactone and h2o2 per min at ph 5.1 At 35 °c, equivalent to an o2 uptake of 22.4 µL per min. If the reaction mixture is saturated with oxygen, the activity may increase by up to 100%.General 'molecular weight: 160 kda (gel filtration) pi: 4.2 Extinction coefficient: e1% = 16.7 (280 Nm)glucose oxidase from aspergillus niger is a dimer consisting of 2 equal subunits with a molecular mass of 80 kda each. Each subunit contains one flavin adenine dinulceotide moiety and one iron. The enzyme is a glycoprotein containing ~16% neutral sugar and 2% amino sugars. The enzyme also contains 3 cysteine residues and 8 potential sites for n-linked glycosylation.Glucose oxidase is capable of oxidizing d-aldohexoses, monodeoxy-d-glucoses, and methyl-d-glucoses at varying rates.The ph optimum for glucose oxidase is 5.5, While it has a broad activity range of ph 4-7. Glucose oxidase is specific for ß-d-glucose with a km of 33-110 mm.Glucose oxidase does not require any activators, but it is inhibited by ag , hg2 , cu2 , phenylmercuric acetate, and p-chloromercuribenzoate. It is not inhibited by the nonmetallic sh reagents: n-ethylmaleimide, iodoacetate, and iodoacetamide.Glucose oxidase can be utilized in the enzymatic determination of d-glucose in solution. As glucose oxidase oxidizes ß-d-glucose to d-gluconolactate and hydrogen peroxide, horseradish peroxidase is often used as the coupling enzyme for glucose determination. Although glucose oxidase is specific for ß-d-glucose, solutions of d-glucose can be quantified as a-d-glucose will mutorotate to ß-d-glucose as the ß-d-glucose is consumed by the enzymatic reaction.Type: type x-sform: lyophilized powder (tlc)composition: protein, 65-85%foreign activity: catalase =5 units/mg proteinstorage temp.: -20°C
