High-Capacity Cdna Reverse Transcription Kit, Delivers Extremely High-Quality, Single-Stranded Cdna From 0.02 To 2μG Total Rna, Each

Details:

The high capacity cdna reverse transcription kit contains all components necessary for the quantitative conversion of up to 2μg of total rna to single-stranded cdna in single 20μl reaction. Reactions can be scaled up to 100μl to generate 10μg of cdna from single reaction. Downstream applications include real-time pcr, standard pcr, and microarrays. The kit is ideal for generating cdna archives.Linear target amplification for real-time pcrhigher yields and precision than other cdna synthesis kits at a fraction of the costten-fold greater dynamic range than other kitsoptimal reaction temperature: 37°cfinal product size: 7kb or lessextensively tested with a variety of templatesquantitative first-strand synthesis of all rna species is achieved with the use of the random primers. The kit has been extensively tested with a variety of rna templates, including gc- and au-rich targets and rnas expressed at low levels. Crna can also be efficiently generated from in vitro transcription of cdnapcr & real-time pcr, reverse transcriptio