Hinfi, Hc (50 U/ΜL), Fermentas Hinfi Is Available As A Fastdigest Enzyme Which Is Used For Rapid Dna Digestion, Each

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Thermo scientific™ hinfi is available as a fastdigest enzyme which is used for rapid dna digestion. More than 95% of the digested dna fragments can be ligated and recut at a 5′-termini concentration of 1.3μM after a 50-fold overdigestion (3u/μg dna x 17 hours) with hinfi.5'...G▵a n t c...3'3'...C t n a▵g...5' Conditions for 100% activity: 1x buffer r: 10mm tris-hcl (ph8.5 At 37°c), 10mm mgcl2, 100mm kcl and 0.1Mg/ml bsa incubate at 37°cstorage buffer: hinfi is supplied in: 10mm tris-hcl (ph 7.4 At 25°c), 100mm kcl, 1mm dtt, 1mm edta, 0.2Mg/ml bsa and 50% (v/v) glycerolmethylation effects: dam: never overlaps —no effect dcm: never overlaps —no effect cpg: may overlap —cleavage impaired ecoki: never overlaps —no effect ecobi: may overlap —blockedcompatible ends: g▴awtc — pfei