Human Egf, Recombinant Protein, Recombinant Protein, Each

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Epidermal growth factor recombinant protein, rp-10927, from invitrogen.Egf (epidermal growth factor) exerts its actions by binding to the egf receptor, a 170 kda protein kinase. Activation of egfr initiates diverse cellular pathways in response to toxic environmental stimuli, or to egf binding to the receptor, the egfr forms homo- or heterodimers with other family members. Each dimeric receptor complex initiates a distinct signaling pathway by recruiting different src homology 2 (sh2) containing effector proteins. Egf is far and wide expressed in kidney, cerebrum, prostrate and salivary glands. Egf acts as a potent mitogenic factor and the phosphorylated receptor recruits adapter proteins like grb2 that activates complex downstream signaling cascades. Egf activates at least 4 major downstream signaling cascades including the ras-raf-mek-erk, pi3 kinase-akt, plcgamma-pkc and stat modules. Research studies suggest the protein may also play important role in activating the nf-kappa-b signaling cascade. Defects in the eggf gene are the cause of hypomagnesemia type 4 and dysregulation has been associated with the growth and progression of certain cancers. This is a recombinant protein suitable for use as a control in elisa and western blot applications. This protein is reported to have the additional biological activity: ed50 as determined by the dose-dependent proliferation of mouse 3t3 cells <0.1Ng/ml. Lyophilized product is stable at room temperature for 3 weeks. Store lyophilized product desiccated at -20°c. Upon reconstitution, store at 4°c for 2-7 days or aliquot and store at -20°c. For long-term storage add either hsa or bsaq to 0.1%. Avoid repeated freeze-thaw cycles. Reconstitute using distilled water to a concentration not less than 100 ug/ml.